IN VIVO LABELING OF MAMMALIAN CONE PHOTORECEPTORS

专利名称：IN VIVO LABELING OF MAMMALIAN CONE

PHOTORECEPTORS BY INTRAVITREALINJECTION OF FLUORESCENTLY TAGGEDPEANUT AGGLUTININ

发明人：DHINGRA, Narender, K.,VIDHYASANKAR, K.申请号：EP08763745.0申请日：20080521公开号：EP2201379B1公开日：20150121

摘要：A method for specifically labeling outer and inner segments and pedicles ofmammalian cone photoreceptors in vivo comprising Intravitreal injection of 2 μl for adultanesthestized mouse (C57BL/6 J), 10 μl for guinea pig (Duncan Hartley) of 20 μl formonkey (Macaca mulatta) of PNA conjugated with a fluorescent probe (Rhodamine orFluorescein) at a concentration of 0.005%, 0.01%, 0.02%, 0.05% or 0.5% (only 0.5% forguinea pig and monkey) with a glass electrode with tip diameter of ~ 1 μm or an insulinsyringe posterior to the limbus into the vitreous of the eye, Removing the eye underanesthesia at various time points (mouse: 0.5, 1, 2, 4 and 8 hours; 1, 2, 4, 7 and 10 days; 1and 3 months; guinea pig and monkey: 30 minutes) after the PNA injection, Hemisectingand fixing the posterior eye-cup in 4% paraformaldehyde for 1 hour at 4°C, Flat mountingthe retina on a glass slide or cryosectioning, and observing PNA labeling with afluorescence microscope.

申请人：DEPT OF BIOTECHNOLOGY INDIA,NAT BRAIN RES CT

地址：IN,IN

国籍：IN,IN

代理机构：Tuxworth, Pamela M.

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